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廖飞老师介绍
廖飞（1969.4～），男；理学硕士，2005年晋升教授，博士生导师；“重庆市高校优秀中青年骨干教师”称号获得者；重庆市第二届学术与技术带头人后备人选；重庆市生物化学与分子生物学会理事；重庆生物化学与分子药理学重点实验室副主任； Clin Biochem、Anal Biochem、Anal Chim Acta等杂志审稿专家。
Tel：13635498457, +23-68486509(H),
E-mail：liaofeish@vip.sina.com
或 liaofeish@cqmu.edu.cn
学习和工作经历
1986~1993，兰州大学生物系，生物化学理学学士和生物物理学理学硕士；
1993.7~2006.12，重庆医科大学生物化学教研室，2001年副教授，2005年教授；
2003~2004，中科院上海药物所新药研究国家重点实验室，学习结构生物信息学；
2007.1~至今，重庆医科大学药学院重庆生物化学与分子药理学重点实验室；
主要教学：硕士生《生物信息学》和部分讲座；参编本科教材一章；
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主持的科研项目
2002~2005年，国家自然科学基金“酶反应动力学过程分析的应用”，编号30200266；19万元
2005~2007年，国家自然科学基金“混合物中靶蛋白高亲合力配体集群筛选方法的建立和应用”，编号 30472139; 20万元；
2007~2009年，国家自然科学基金“苛求芽孢杆菌胞内尿酸酶的克隆表达、分子改造和应用”，编号30672009; 28万元；
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研究方向：蛋白质(酶)的配体设计与筛选；蛋白质(酶)分子工程和应用；
主要技术：光谱分析与色谱分析技术；基因克隆与定点突变技术；化学计量学与酶动力学数据分析技术；蛋白质化学修饰与固定化技术；
主要应用：配体类药物设计与筛选；生物技术药物设计与制剂；药物药效学与毒理学初步评价；酶催化不对称合成手性化合物；数据分析与决策判定；
基本要求：药学、生物学或化学相关专业背景；发表过CSCD核心刊物研究论文；
其它要求：积极上进；认真负责；吃苦耐劳；有较好专业外语基础；
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作为通讯作者发表的代表论文和第一发明人申报(或授权)的发明专利
1. 廖飞，张晓萍，周岐新，曾昭淳，陈文缘。 离子交换高效液相层析测定腺苷酸环化酶活性。 生物化学与生物物理学报 ，2000；32 :661-664 (SCI，IF>0.30).
2. Fei Liao, Wan-Li Liu, Qi-xin Zhou, Zhao-Chun Zeng, Yu-Ping Zuo. Assay of serum arylesterase activity by fitting to the reaction curve with an integrated rate equation. Clin Chim Acta, 2001；314: 67-76 (SCI, IF >1.30).
3. Fei Liao, Kao-Cong Tian, Xiao Yang, Qi-Xin Zhou, ?Zhao-Chun Zeng, Yu-Ping Zuo. Kinetic substrate quantification by fitting to the integrated Michaelis-Menten equation. Anal Bioanal Chem, 2003；375:756-762 (SCI, IF>1.75).
4. Fei Liao, Jiachu Li, Gefei Kang, Zhaochun Zeng, Yuping. Zuo. Measurement of mouse liver glutathione-S- transferase activity by the integrated method. J Med Coll PLA, 2003；18(5):295-300.
5. Fei Liao, Xiao Yang, Qixin Zhou, Zhaochun Zeng, Yuping Zuo. Fast estimation of the Michaelis-Menten constant of arylesterase with a pair of medium concentrations of substrate. J Med Coll PLA, 2003；18(5):312-316.
6. Fei Liao, XiaoYun Zhu, YongMei Wang, YuPing Zuo. The comparison on the estimation of kinetic parameters by fitting enzyme reaction curve to the integrated rate equation of different predictor variables. J Biochem Biophys Methods, 2005；62(1): 13-24 (SCI, IF >1.3).
7. Fei Liao，YunSheng Zhao，Lina Zhao，Jia Tao，Xiaoyun Zhu，Yongmei Wang，Yuping Zuo. Direct kinetic assay of substance in body fluids by predicting background with the integrated method using uricase as model. J Med Coll PLA 2005; 20(6): 338-344.
8. Fei Liao，YunSheng Zhao，Lina Zhao，Jia Tao，Xiaoyun Zhu，Lan Liu. The evaluation of a direct kinetic method for serum uric acid assay by predicting the background absorbance of uricase reaction solution with an integrated method. J Zhejiang Univ Sci B, 2006；7(6): 497-502. 9. Yunsheng Zhao,Lina Zhao, Gengqing Yang, Jia Tao, Youquan Bu, Fei Liao. Characterization of an intracellular uricase from Bacillus fastidious ATCC 26904 and its application to serum uric acid assay by a patented kinetic method. Biotechnol Appl Biochem, 2006；45(2):75-80 (SCI，IF>1.89).
10. Fei Liao, Lina Zhao, Yunsheng Zhao, Jia Tao, Yuping Zuo. Integrated rate equation considering product inhibition and its application to kinetic assay of serum ethanol. Anal Sci, 2007; 23(4):439-444 (SCI，IF>1.69).
11. Fei Liao, Xiao-yun Zhu, Yong-mei Wang, Yun-sheng Zhao, Lian-ping Zhu, Yu-ping Zuo. Correlation of serum arylesterase activity on phenylacetate estimated by the integrated method to common classical biochemical indexes of liver damage. J Zhejiang Univ Sci B, 2007；8(4):237-241.
12. Rui Guo, shengbing Li, Lina Zhao, Yunsheng Zhao, Wei Lu, Pei Yuan, Ping Deng, Fei Liao. A new linearly-combined bi-exponential model for kinetic analysis of the isometric relaxation process of Bufo gastrocnemius under electric stimulation in vitro. J Zhejiang Univ Sci B, 2007；8(12):867-873.
13. Zhenli Cheng, Hong Chen, Yunsheng Zhao, Xiaolan Yang, Wei Lu, Hong Liao, Ming’an Yu, Fei Liao. The measurement of the activity of rabbit muscle lactic dehydrogenase by integrating the classical initial rate method with an integrated method. 2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008, (art. no. 4535510), pp. 1209-1212. Shanghai, 2008 (EI)
14. Yunsheng Zhao, Xiaolan Yang, Fei Liao. Uricase based method for determination of uric acid in serum. Microchim Acta, 2009；164(1):1-6. (SCI, IF>1.2).
15. Beizhong Liu, Yunsheng Zhao, Lina Zhao, Yanlin Xie, Sha Zhu, Zhirong Li, Yin Liu, Wei Lu, Xiaolan Yang, Guoming Xie, Huansi Zhong, Mingan Yu, Hong Liao, Fei Liao. An integration strategy to estimate the initial rates of enzyme reactions with much expanded linear ranges using uricases as models. Anal Chim Acta, 2009；631: 22-28 (SCI, IF > 3.1).
16. Sha Zhu, Zhiyong Gan, Zhirong Li, Yin Liu, Xiaolan Yang, Ping Deng, Yanlin Xie, Mingan Yu, Hong Liao,Yunsheng Zhao, Lina Zhao, Fei Liao. The measurement of cyclic nucleotide phosphodiesterase 4 activities via the quantification of inorganic phosphate with malachite green. Anal Chim Acta 2009；636:105-110 (SCI, IF > 3.1).
17. Fei Liao, Diyuan Yang, Jingqun Tang, Xiaolan Yang, Beizhong Liu, Yunsheng Zhao, Lina Zhao, Hong Liao, Ming'an Yu. The measurement of serum cholinesterase activities by an integration strategy with expanded linear ranges and negligible substrate-activation. Clin Biochem; 2009；(http://dx.doi.org/10.1016/j.clinbiochem.2008.11.016) (SCI, IF > 2.0).
18. Diyuan Yang, Jingqun Tang, Xiaolan Yang, Ping Deng, Yunsheng Zhao, Sha Zhu, Yanling Xie, Xinbi Dai, Hong Liao, Ming’an Yu, Juan Liao, Fei Liao. An Integration Strategy to Measure Enzyme Activities for Detecting Irreversible Inhibitors with Dimethoate on Butyrylcholinesterase as Model. Int J Environ Anal Chem, 2009；in press; (SCI, IF > 1.0).
19. 廖飞。 通过预测本底进行酶法分析定量测定体液尿酸含量的方法。 中国发明专利 ZL 03135649.4, 授权日期2005-08-31。
20. 廖飞，赵运胜，赵利娜，陆巍，杨晓兰，廖红，于明安，桑宇. 联用积分法和初速度法测定酶活性的方法。中国发明专利，公开号CN101173889A。
21. 廖飞，杨晓兰，谢燕玲，邓萍，赵运胜，朱莎，甘志勇，廖娟，刘冬，于明安。联用磁分离与色谱分析测定混合物中配体亲和力的方法。中国发明专利，受理号 200810237298.2.
22. 廖飞，邓萍，谢燕玲，杨晓兰，赵运胜，朱莎，廖娟，刘冬，于明安。以蛋白中色氨酸残基为Forster共振能量转移供体的均相亲和分析方法。中国发明专利，受理号 200810237000.8.
Tel：13635498457, +23-68486509(H),
E-mail：liaofeish@vip.sina.com
或 liaofeish@cqmu.edu.cn
学习和工作经历
1986~1993，兰州大学生物系，生物化学理学学士和生物物理学理学硕士；
1993.7~2006.12，重庆医科大学生物化学教研室，2001年副教授，2005年教授；
2003~2004，中科院上海药物所新药研究国家重点实验室，学习结构生物信息学；
2007.1~至今，重庆医科大学药学院重庆生物化学与分子药理学重点实验室；
主要教学：硕士生《生物信息学》和部分讲座；参编本科教材一章；
--------------------------------------------------------------------------------
主持的科研项目
2002~2005年，国家自然科学基金“酶反应动力学过程分析的应用”，编号30200266；19万元
2005~2007年，国家自然科学基金“混合物中靶蛋白高亲合力配体集群筛选方法的建立和应用”，编号 30472139; 20万元；
2007~2009年，国家自然科学基金“苛求芽孢杆菌胞内尿酸酶的克隆表达、分子改造和应用”，编号30672009; 28万元；
--------------------------------------------------------------------------------
研究方向：蛋白质(酶)的配体设计与筛选；蛋白质(酶)分子工程和应用；
主要技术：光谱分析与色谱分析技术；基因克隆与定点突变技术；化学计量学与酶动力学数据分析技术；蛋白质化学修饰与固定化技术；
主要应用：配体类药物设计与筛选；生物技术药物设计与制剂；药物药效学与毒理学初步评价；酶催化不对称合成手性化合物；数据分析与决策判定；
基本要求：药学、生物学或化学相关专业背景；发表过CSCD核心刊物研究论文；
其它要求：积极上进；认真负责；吃苦耐劳；有较好专业外语基础；
--------------------------------------------------------------------------------
作为通讯作者发表的代表论文和第一发明人申报(或授权)的发明专利
1. 廖飞，张晓萍，周岐新，曾昭淳，陈文缘。 离子交换高效液相层析测定腺苷酸环化酶活性。 生物化学与生物物理学报 ，2000；32 :661-664 (SCI，IF>0.30).
2. Fei Liao, Wan-Li Liu, Qi-xin Zhou, Zhao-Chun Zeng, Yu-Ping Zuo. Assay of serum arylesterase activity by fitting to the reaction curve with an integrated rate equation. Clin Chim Acta, 2001；314: 67-76 (SCI, IF >1.30).
3. Fei Liao, Kao-Cong Tian, Xiao Yang, Qi-Xin Zhou, ?Zhao-Chun Zeng, Yu-Ping Zuo. Kinetic substrate quantification by fitting to the integrated Michaelis-Menten equation. Anal Bioanal Chem, 2003；375:756-762 (SCI, IF>1.75).
4. Fei Liao, Jiachu Li, Gefei Kang, Zhaochun Zeng, Yuping. Zuo. Measurement of mouse liver glutathione-S- transferase activity by the integrated method. J Med Coll PLA, 2003；18(5):295-300.
5. Fei Liao, Xiao Yang, Qixin Zhou, Zhaochun Zeng, Yuping Zuo. Fast estimation of the Michaelis-Menten constant of arylesterase with a pair of medium concentrations of substrate. J Med Coll PLA, 2003；18(5):312-316.
6. Fei Liao, XiaoYun Zhu, YongMei Wang, YuPing Zuo. The comparison on the estimation of kinetic parameters by fitting enzyme reaction curve to the integrated rate equation of different predictor variables. J Biochem Biophys Methods, 2005；62(1): 13-24 (SCI, IF >1.3).
7. Fei Liao，YunSheng Zhao，Lina Zhao，Jia Tao，Xiaoyun Zhu，Yongmei Wang，Yuping Zuo. Direct kinetic assay of substance in body fluids by predicting background with the integrated method using uricase as model. J Med Coll PLA 2005; 20(6): 338-344.
8. Fei Liao，YunSheng Zhao，Lina Zhao，Jia Tao，Xiaoyun Zhu，Lan Liu. The evaluation of a direct kinetic method for serum uric acid assay by predicting the background absorbance of uricase reaction solution with an integrated method. J Zhejiang Univ Sci B, 2006；7(6): 497-502. 9. Yunsheng Zhao,Lina Zhao, Gengqing Yang, Jia Tao, Youquan Bu, Fei Liao. Characterization of an intracellular uricase from Bacillus fastidious ATCC 26904 and its application to serum uric acid assay by a patented kinetic method. Biotechnol Appl Biochem, 2006；45(2):75-80 (SCI，IF>1.89).
10. Fei Liao, Lina Zhao, Yunsheng Zhao, Jia Tao, Yuping Zuo. Integrated rate equation considering product inhibition and its application to kinetic assay of serum ethanol. Anal Sci, 2007; 23(4):439-444 (SCI，IF>1.69).
11. Fei Liao, Xiao-yun Zhu, Yong-mei Wang, Yun-sheng Zhao, Lian-ping Zhu, Yu-ping Zuo. Correlation of serum arylesterase activity on phenylacetate estimated by the integrated method to common classical biochemical indexes of liver damage. J Zhejiang Univ Sci B, 2007；8(4):237-241.
12. Rui Guo, shengbing Li, Lina Zhao, Yunsheng Zhao, Wei Lu, Pei Yuan, Ping Deng, Fei Liao. A new linearly-combined bi-exponential model for kinetic analysis of the isometric relaxation process of Bufo gastrocnemius under electric stimulation in vitro. J Zhejiang Univ Sci B, 2007；8(12):867-873.
13. Zhenli Cheng, Hong Chen, Yunsheng Zhao, Xiaolan Yang, Wei Lu, Hong Liao, Ming’an Yu, Fei Liao. The measurement of the activity of rabbit muscle lactic dehydrogenase by integrating the classical initial rate method with an integrated method. 2nd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008, (art. no. 4535510), pp. 1209-1212. Shanghai, 2008 (EI)
14. Yunsheng Zhao, Xiaolan Yang, Fei Liao. Uricase based method for determination of uric acid in serum. Microchim Acta, 2009；164(1):1-6. (SCI, IF>1.2).
15. Beizhong Liu, Yunsheng Zhao, Lina Zhao, Yanlin Xie, Sha Zhu, Zhirong Li, Yin Liu, Wei Lu, Xiaolan Yang, Guoming Xie, Huansi Zhong, Mingan Yu, Hong Liao, Fei Liao. An integration strategy to estimate the initial rates of enzyme reactions with much expanded linear ranges using uricases as models. Anal Chim Acta, 2009；631: 22-28 (SCI, IF > 3.1).
16. Sha Zhu, Zhiyong Gan, Zhirong Li, Yin Liu, Xiaolan Yang, Ping Deng, Yanlin Xie, Mingan Yu, Hong Liao,Yunsheng Zhao, Lina Zhao, Fei Liao. The measurement of cyclic nucleotide phosphodiesterase 4 activities via the quantification of inorganic phosphate with malachite green. Anal Chim Acta 2009；636:105-110 (SCI, IF > 3.1).
17. Fei Liao, Diyuan Yang, Jingqun Tang, Xiaolan Yang, Beizhong Liu, Yunsheng Zhao, Lina Zhao, Hong Liao, Ming'an Yu. The measurement of serum cholinesterase activities by an integration strategy with expanded linear ranges and negligible substrate-activation. Clin Biochem; 2009；(http://dx.doi.org/10.1016/j.clinbiochem.2008.11.016) (SCI, IF > 2.0).
18. Diyuan Yang, Jingqun Tang, Xiaolan Yang, Ping Deng, Yunsheng Zhao, Sha Zhu, Yanling Xie, Xinbi Dai, Hong Liao, Ming’an Yu, Juan Liao, Fei Liao. An Integration Strategy to Measure Enzyme Activities for Detecting Irreversible Inhibitors with Dimethoate on Butyrylcholinesterase as Model. Int J Environ Anal Chem, 2009；in press; (SCI, IF > 1.0).
19. 廖飞。 通过预测本底进行酶法分析定量测定体液尿酸含量的方法。 中国发明专利 ZL 03135649.4, 授权日期2005-08-31。
20. 廖飞，赵运胜，赵利娜，陆巍，杨晓兰，廖红，于明安，桑宇. 联用积分法和初速度法测定酶活性的方法。中国发明专利，公开号CN101173889A。
21. 廖飞，杨晓兰，谢燕玲，邓萍，赵运胜，朱莎，甘志勇，廖娟，刘冬，于明安。联用磁分离与色谱分析测定混合物中配体亲和力的方法。中国发明专利，受理号 200810237298.2.
22. 廖飞，邓萍，谢燕玲，杨晓兰，赵运胜，朱莎，廖娟，刘冬，于明安。以蛋白中色氨酸残基为Forster共振能量转移供体的均相亲和分析方法。中国发明专利，受理号 200810237000.8.
廖飞老师相关教学资源
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廖飞老师教学评价(4条)
我要评论(500字以内)
举报wedslyxdbp 2021/3/10 21:54:56
廖飞教授，你好。我曾老师学生朱海兵，我没曾昭淳教授联系方式了，如果您知道麻烦你能告知我吗？我的电话18973397645
沟通交流、共同成长。欢迎您参与评论留言！
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