开国银 副教授
　
开国银 博士，副教授，硕士生导师，上海师范大学植物生物工程实验室负责人，上海市遗传学会理事，上海市植物生理学会会员，中国遗传学会会员，江苏省药用植物生物技术重点实验室兼职教授。主要从事植物分子生物学的研究。2005年毕业于上海交通大学获生物化学与分子生物学专业博士学位,并于2005年6月破格晋升为副教授。
2000年至2002年，曾在复旦大学遗传工程国家重点实验室客座研究，经受过系统的基因工程技术训练，在基因克隆、载体构建、遗传转化、表达调控和代谢分析等方面具有扎实的理论基础和丰富的实践经验。攻读博士期间曾荣获“国家优秀奖学金”等奖励，2005年3月被授予“上海市优秀毕业生”称号。2006年入选“上海市青年科技启明星”人才计划。2007年被评为“上海师大优秀青年教师”。先后主持或参与多项科研课题的攻关研究，具有良好的科研创新能力及团队合作精神！
近期在国内外期刊如PHYSIOLOGIA PLANATARUM, JOURNAL OF BIOCHEMSITRY AND MOLECULAR BIOLOGY, PLANT SCIENCE, BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, JOURNAL OF BIOSCIENCE, BIOLOGIA PLATARUM, MOLECULAR BIOLOGY REPORTS, DNA SEQUENCE, BIOLOGIA, JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY，ACTA PHYSIOLOGIAE PLANTARUM, PLANTA及PNAS等上发表生物技术领域文章40多篇，其中SCI收录26篇，申请中国发明专利8项（授权3项），在国际GenBank登录全长新基因30多条,并担任SCI期刊《Bioresource Technology》,《Cell Biology International》和《Journal of Integrative Plant Biology》等的审稿专家。
近期发表的主要论文如下(*通讯作者)：
Guoyin Kai*, Yan Zhang, Junfeng Chen, Li Li, Xiangming Yan, Ran Zhang, Pan Liao, Xuan Lu, Wei Wang, Genyu Zhou. Molecular Characterization and Expression Analysis of Two Distinct Putrescine N-methyltransferases from Roots of Anisodus acutangulus. Physiologia Planatarum. 2008 (accepted on 13 October, SCI Journal)
Yang Lu, Hongshen Wang, Wei Wang, Zhongying Qian, Li Li, Jing Wang, Genyu Zhou, Guoyin Kai*. Molecular Characterization and Expression Analysis of a New cDNA Encoding Strictosidine Synthase from Ophiorrhiza japonica. Molecular biology reports 2008 (accepted on 17 October, online published on 6 November, SCI Journal)
Pan Liao, Wei Zhou, Lin Zhang, Jing Wang, Xiangming Yan, Yan Zhang, Ran Zhang, Li Li, Genyu Zhou, Guoyin Kai*. Molecular Cloning, characterization and expression analysis of a new gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase from Salvia miltiorrhiza. Acta Physiologiae Plantarum, 2008 (accepted on 23 November, SCI Journal)
Guoyin Kai*, Liming Dai, Xinyuan Mei, Jingui Zheng, Wei Wang, Yang Lu, Zhongying Qian, Genyu Zhou. In vitro plant regeneration from leaf explants of Ophiorrhiza japonica. Biologia Plantarum 2008, 52 (3): 557-560 (SCI Journal)?
Li Li, Jing Wang, Wei Wang, Yang Lu, Yuliang Wang, Genyu Zhou, Guoyin Kai*. Optimization of induction and culture conditions and tropane alkaloid production in hairy roots of Anisodus acutangulus. Biotechnology and Bioprocess Engineering 2008, 13: 606-612 (SCI Journal)
Guoyin Kai*, Junfeng Chen, Li Li, Genyu Zhou, Limin Zhou, Lei Zhang, Yuhui Chen, Linxia Zhao. Molecular cloning and characterization of a new cDNA encoding hyoscyamine 6β-hydroxylase from roots of Anisodus acutangulus. Journal of Biochemistry and Molecular Biology,2007,40(5): 715- 722 (SCI Journal)???
Binhui Guo+,Guoyin Kai+,Yifu Gong,Hongbin Jin, Yechun Wang, Zhiqi Miao, Xiaofen Sun, Kexuan Tang. Molecular cloning and heterologous expression of a 10-deacetylbaccatin III-10-O-acetyl transferase cDNA from Taxus x media. Molecular biology reports 2007,34:89-95 (co-first author, SCI Journal)???
Guoyin Kai*, Yang Lu, Zhongying Qian, Yongting Luo, Genyu Zhou, Kexuan Tang. Molecular characterization and expression analysis of a gene encoding mannose -binding lectin from bulb of Zephyranthes grandiflora. Biologia－section Cellular and Molecular Biology 2006，61(6)：671-677(SCI Journal)
Guoyin Kai, Zhiqi Miao, Lei Zhang, Dongli Zhao, Zhihua Liao, Xiaofen Sun, Lingxia Zhao, Kexuan Tang. Molecular cloning and expression analyses of a new gene encoding 3-hydroxy-3-methylglutaryl -CoA synthase from Taxus x media. Biologia Plantarum 2006,50 (3): 359-366(SCI Journal)?????????
Guoyin Kai, Jihong Jiang, Dongli Zhao, Lingxia Zhao, Lei Zhang, Zhugang Li, Binhui Guo, Xiaofen Sun, Zhiqi Miao, Kexuan Tang. Isolation and expression profile analysis of a new cDNA encoding 5-alpha-taxadienol-10-beta-hydroxylase from Taxus media. Journal of Plant Biochemistry and Biotechnology 2006,15(1): 1-5 (SCI Journal)
Guoyin Kai, Lingxia Zhao, Lei Zhang, Zhugang Li, Binhui Guo, Dongli Zhao, Xiaofen Sun, Zhiqi Miao, Kexuan Tang. Characterization and expression profile analysis of a new cDNA encoding taxadiene synthase from Taxus media. Journal of Biochemistry and Molecular Biology 2005,38(6): 675-685 (SCI Journal)
Lei Zhang+, Guoyin Kai+, Beibei Lu, Hanming Zhang, Kexuan Tang, Jihong Jiang, Wansheng Chen. Metabolic Engineering of Tropane Alkaloid Biosynthesis in Plants. Journal of Integrative Plant Biology 2005, 47(2): 136-143 (SCI Journal，co-first author)
Guoyin Kai, Zhiqi Miao, Chengxiang Qiu, Lei Zhang, Lingxia Zhao, Zhugang Li, Tiefeng Xu, Lida Zhang, Yifu Gong, Dongli Zhao, Donghui Liu, Xiaofen Sun, Kexuan Tang. Molecular cloning and characterization of a taxadienol acetyl transferase cDNA from Taxus media. Plant science 2004,167(4):759-764(SCI Journal)
Guoyin Kai, Lingxia Zhao, Jingui Zheng, Lei Zhang, Zhiqi Miao, Xiaofen Sun, Kexuan Tang. Isolation and characterization of a new mannose-binding lectin gene from Taxus media. Journal of Biosciences 2004,29(4):399-407 (SCI Journal)
Guoyin Kai, Jingui Zheng, Lei Zhang, Yongzheng Pang, Zhihua Liao, Zhugang Li, Lingxia Zhao, Xiaofen Sun, Kexuan Tang. Molecular Cloning of a New Lectin Gene from Z. grandiflora. DNA Sequence 2003,14(4):335-338 (SCI Journal)
Guoyin Kai, Lei Zhang, Hongyu Zhang, Tiefeng Xu, Kexuan Tang, Hanming Zhang. Marker free: a novel tendency of transgenic plants. Acta Botanica Sinica 2002,44(8):883–888 (SCI Journal)
Zhihua Liao, Yifu Gong, Guoyin Kai, Kaijin Zuo, Min Chen, Qiumin Tan, Yamin Wei,Liang Guo,Feng Tan, Xiaofen Sun, Kexuan Tang. An Intron-free Methyl Jasmonate Inducible Geranylgeranyl Diphosphate Synthase Gene from Taxus media and its functional identification in yeast. Molecular Biology 2005，39(1):14-20.(SCI Journal)?
Jihong Jiang, Guoyin Kai, Xiaoyin Cao, Fengmei Chen, Dongning He, Qun Liu. Molecular cloning and function identification of a new HMG-CoA reductase gene from Eucommia ulmoides Olive. Bioscience reports 2006,26(2):171-181(SCI Journal)
Lei Zhang, Guoyin Kai, Tiefeng Xu, Yan Pi, Hanming Zhang, Xiaofen Sun, Kexuan Tang. Efficient regeneration of tetraploid Isatis indigotica plants via adventitious organogenesis from hypocotyl explants. Biologia Plantarum, 2004, 48(1):121-124 (SCI Journal)
Binghui Guo, Guoyin Kai, Hongbing Jin, Kexuan Tang. Taxol synthesis. African Journal of Biotechnology.2006,5(1):15-20
Zhugang Li, Lingxia Zhao, Guoyin Kai, Shunwu Yu, Youfang Cao, Yongzhen Pang, Xiaofen Sun, Kexuan Tang. Cloning and expression analysis of a water stress-induced gene from Brassica oleracea. Plant physiology and biochemistry. 2004, 42(10): 789-794.(SCI Journal)
Zhugang Li, Chongshi Cui,Lingxia Zhao, Guoyin Kai, Lida Zhang, Xiaofen Sun, Kexuan Tang. Molecular cloning and characterization of an anti-bolting related gene (BcpFLC3) from Brassica campestris ssp.Pekinensis. Plant Science 2005, 168: 407-413 (SCI Journal)
Lingxia Zhao, Chengxiang Qiu, Jingfu Li, Yourong Chai, Guoyin Kai, Zhugang Li, Xiaofen Sun, Kexuan Tang. Investigation of disease resistance and cold tolerance of Solanum lycopersicoides for tomato improvement. HortScience 2005, 40(1):43-46 (SCI Journal)
Yifu Gong, Zhihua Liao, Min Chen, Kaijin Zuo, Liang Guo, Qiumin Tan, Zhushi Huang, Guoyin Kai, Xiaofen Sun, Feng Tan, Kexuan Tang. Molecular cloning and characterization of a 1-deoxy-D-xylulose 5-phosphate reductoisomerase gene from Ginkgo biloba. DNA Sequence 2005，16(2):111-120 (SCI Journal)
Dongli Zhao, Le Li, Qian Wang, Guoyin Kai, Xiang Wang, Lu Wang, Xiaofen Sun, Kexuan Tang. Cloning of taxane 2α-O-benzoyltransferase (TBT) genomic DNA fromTaxus. Biologia-Section Cellular and Molecular Biology 2006,61(3): 327-329 (SCI Journal)
Linxia Zhao, Jingfu Li, Yourong Chai, Guoyin Kai, Youfang Cao, Xiaofen Sun, Kexuan Tang. Investigation on genetic relationship and cross compatibility of S.lycopersicoides and lycopersicno. Pakistan Journal of Biological Sciences. 2006,9(6):1160-1168
Lei Zhang, Ruxian Ding, Yourong Chai, Mercedes Bonfill, M. Teresa Pi?ol, Tiefeng Xu, Yan Pi, Zinan Wang, Hanming Zhang, Guoyin Kai, Zhihua Liao, Xiaofen Sun, Kexuan Tang. Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures. Proc Natl Acad Sci U S A，2004, 101(17):6786-6791. (SCI Journal)
Zhang Lei, Huang BeiBei, Kai GuoYin, Guo MeiLi. Analysis of inter-population genetic variation in Chinese Carthamus tinctorius L. using AFLP markers. Acta Pharmaceutica Sinica 2006,41(1):91-96
Dongli Zhao, Qian Wang, Guoyin Kai, Xiang Wang,, Juan Lin, Yan Pi, Ziqi Miao, Xiaofen Sun, Kexuan Tang. Molecular cloning and characterization of cDNA encoding taxane 2α-O-benzoyltransferase (TBT), catalyzing taxol biosynthesis from Taxus media. Journal of Biological Sciences 2006,6(4)：651－658
Guoyin Kai*, Junfeng Chen, Wei Zhou, Liming Dai, Genyu Zhou. Cloning and Sequence Analysis of a Cold-regulated Gene BnCOR14 from Brassica napus. Acta Bot.Boreal.-Occident.Sin. 2007,27(1): 9-15
Lei Zhang，Bin Yang，Beibei Lu，Guoyin Kai， Zinan Wang，Yang Xia， Ruxian Ding， Hanming Zhang， Xiaofen Sun，Wansheng Chen，Kexuan Tang. Tropane alkaloids production in transgenic Hyoscyamus niger hairy root cultures over-expressing Putrescine N-methyltransferase is methyl jasmonate-dependent. Planta 2007,225(4):887-896 (SCI Journal)
Zhang L, Wang Z, Xia Y, Kai G, Chen W, Tang K. Metabolic engineering of plant L-ascorbic acid biosynthesis: recent trends and applications. Critical Reviews in Biotechnology.2007, 27(3):173－182 (SCI Journal)
陆倍倍, 张磊，开国银，张汉明，丁如贤，陈万生.莨菪发根培养体系的建立.中草药2005,36(12):1864-1868
周伟,沈亚芳,刘材材,戴黎鸣,陈军峰,开国银*,周根余*.稀土微肥对铁皮石斛试管壮苗的影响。中草药，2006，37（11）：13－17
赵凌侠,张凌,魏捷,开国银,李柱刚,钱虹妹,张慧,唐克轩.利用烟草表达人凝血IX因子(hFIX)的研究.中国生物工程杂志,2006,26(3):11-16
戴黎鸣,周伟,陈军峰,开国银*,周根余*.影响大岩桐试管块茎形成的若干因素.上海师范大学学报, 2006,35(5):71-75
周伟,姚倩雯,钱忠英,沈亚芳,刘圆圆,张磊,周根余*,开国银*.丹参毛状根诱导条件的优化.上海师范大学学报, 2007,36(2):93-98
周伟,沈亚芳,陈军峰,戴黎鸣,开国银*,周根余*.丹参生物工程的研究与开发。生物学杂志，2007，24（3）：48－51
陈军峰,张艳,戴黎鸣,周伟,沈婧菁, 开国银*,周根余*.铁皮石斛胚状体液体悬浮培养条件的研究.上海师范大学学报, 2007,36(1):69-73
张艳,钱忠英, 陈军峰,开国银*,周根余*. 香蕉提取物对霍山石斛原球茎形成和小苗生长的影响。上海师范大学学报, 2008，37（4）：415-419
王伟，陆杨，李礼，王敬，开国银*.喜树毛状根的诱导及其喜树碱的产生.西北植物学报，2008，12：(排版中)
发明专利申请：
1.开国银、苗志奇、唐克轩.红豆杉3-羟基-3-甲基戊二酰辅酶A合成酶蛋白编码序列（已授权）专利号ZL200410024891.0
2.开国银，廖攀，周伟, 董彦君，周根余。丹参3-羟基-3-甲基戊二酰辅酶A还原酶基因及其编码的蛋白质和应用。中国专利申请号：200810035721.0
3. 开国银，张艳，陈军峰，张林，董彦君，周根余。三分三1, 4-丁二胺-氮-甲基转移酶基因1及其编码的蛋白质和用途。中国专利申请号：200810035720.6
4.开国银，李礼，王敬，王伟，陆杨，周根余.三分三托品酮还原酶I基因及其编码的蛋白质和应用。中国发明专利申请号200710047000.7
5.开国银，陈军峰，李礼，周伟，周根余.三分三莨菪碱6β-羟化酶蛋白及其编码基因与用途.中国发明专利申请号200610118927.0
6.开国银，王伟，陆杨，张冉，董彦君，周根余。喜树3-羟基-3-甲基戊二酰辅酶A合成酶基因及其编码的蛋白质和应用。中国专利申请号：200810035717.4?
7.唐克轩、开国银、苗志奇、李柱刚、赵凌侠。红豆杉凝集素蛋白编码序列（已授权）专利号ZL200410016709.7
8.唐克轩、开国银、苗志奇、李柱刚、赵凌侠。红豆杉紫杉烷C13－侧链－N－苯甲酰转运酶蛋白编码序列（已授权）专利号ZL200410016769.9
科研项目情况:
1.上海市青年科技启明星计划（06QA14038）:日本蛇根草中喜树碱生物合成的代谢工程研究（2006.6 -2008.12）主持 执行顺利
2.上海市教委科研创新重点项目(09ZZ138)：丹参酮生物合成途径中关键酶基因的克隆及鉴定（2008.9 -2010.12）主持 执行顺利
3.上海市科委西部开发科技合作项目（065458022）：云南省石斛种质资源的收集保护与开发利用（2006.8 -2008.12）主持 执行顺利
4.上海市科委重点科技攻关项目（075405117）：富含γ-氨基丁酸的功能性稻米品种选育及其生物合成的分子调控研究（2007.12-2010.12）子课题负责人 执行顺利
5.上海市科委振兴东北科技合作项目（073158202）：多功能樱桃番茄新品种的培育（2007.9-2009.12）子课题负责人 执行顺利
6.上海师范大学一般科研项目（SK200830）：莨菪生物碱代谢合成途径中关键酶基因的克隆及应用（2008.6-2010.6）主持 执行顺利
7.上海市科委重点科技攻关项目“青蒿药物植物产品关键技术研究与应用（08391911800）” （2008.7-2011.9） 子课题负责人 执行顺利
8.上海市自然科学基金项目（05ZR14093）:利用基因工程技术提高三分三中东莨菪碱含量的研究（2005.9-2007.12） 主持 顺利完成
　
　
联系方式：
电子邮件：gykai@shnu.edu.cn