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陈德喜
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所在大学:首都医科大学
所在院系:佑安医院
所在地区:北京
所在城市:北京
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陈德喜老师介绍
1982.9-1987.7:包头医学院医学系本科。
1990.9-1993.7: 包头医学院生化硕士研究生(1990-1991 在首都医科大学学习基础课)。
1994.9-1997.7:北京医科大学生物化学与分子生物专业博士。
1997.8-2000.11:博士后学习。美国匹兹堡大学医学院神经系脑缺血分子生物研究室做博士后。主要研究课题是: 一,寻找脑缺血诱导的神经保护性新基因。二,脑缺血后基因修复的分子机制。
2000.12 -2002.8: 助理研究员(Assistant Scientist)俄勒冈州 波特兰市 DowS Neurobiology Labs.负责脑缺血耐受现象的分子生物学机制研究。
2002.9-2006.8: 2002年9月:Research Assistant Professor。受聘于俄勒冈州立医科大学医学院肿瘤研究所,从事肿瘤凋亡调控的分子机制研究。
2006.9-现在:首都医科大学附属北京佑安医院艾滋病研究室主任,教授和博士生导师。
在美国期间先后参与和协作主持了6个NIH基金:
“Inducible DNA Repair in Cerebral Ischemia” R01 2R01NS036736-05 NIH/NINDS, Principal Investigator: Jun Chen, M.D., Ph.D.; Co-Investigator: Dexi Chen, Ph.D. 2000-2005
“Mitochondrial DNA Damage and Repair in Ischemic Brain Injury” R01 1R01NS038560-01 NIH/NINDS, Principal Investigator: Jun Chen, M.D., Ph.D.; Co-Investigator: Dexi Chen, M.D., Ph.D. 1998-2003
“Cell Excitation in Ischemic Brain Injury” RO1 NS24728-14 NIH/NINDS, Principal Investigator: Roger, P Simon, M.D., Ph.D.。2000-2005
"Molecular Mechanisms in Ischemia” 1 PO1 NS35965-01 (Program Project Grant; RP SIMON PI).1997-2002
“A Role of Acid-Sensing Ion Channels in Ischemia” R21 1R21NS042799-01 Exploratory/Developmental Grant Program. Agency: NIH/NINDS, Principal Investigator: Roger P simon, M.D., Ph.D.; 2001-2003
“Mechanisms linking ASPP2 to the Rb/E2F1 and p53 pathway” Ro1CA104997-01. Lopez (PI), Co-Investigator, Dexi chen, M.D., Ph.D. 2004-2009。
北京市科委1400万艾滋病重大课题(D0909003040591)参与者(2006-2009)
Memberships in Professional Organization
Society for Neuroscience 1998-2003
American Association for Cancer Research 2003 – present
1. Dexi Chen, Zhiyong Yu, Zhiyi Zhu, Lopez CD. E2F target to XRCC1and contribution to single strand broken repair in S phase. Will be Presented in AACR 97th annual meeting, 2006, April 1 – 5 Washington DC (Submitted to Cell death and differation)
2. Dexi Chen , Zhiyi Zhu, Zhiyong Yu, Charles D. Lopez . An intact p53 pathway is required for efficient mitochondrial BER. Cancer Research. 2006,66(&):3485-3494.
3. Zhiyi Zhu, Jason Ramos, Kerstin Kampa, Zhiyong Yu, Dexi chen, Charles Lopez. ASPP2 Apoptotic is Modulated by Proteasomal Degradation. J. Biol. Chem. 2005 288(41): 34473-88.
4. Li Ning, Wu Hao, Sufang Yang, Dexi Chen#. Ischemic Preconditioning Induces the Expression of Base Excision Repair (BER) Enzymes- Indicating An Important Role for DNA Repair in Focal Ischemic Tolerance. ( In press J DNA Repair)
5. Chen D, Padiernos E, Ding F, Lossos IS, Lopez CD. Apoptosis-stimulating protein of p53-2 (ASPP2/53BP2L) is an E2F target gene.Cell Death Differ. 2005 Apr;12(4):358-68
6. Meller R, Minami M, Cameron J, Chen D, Lan J, HenshaLL D, SIMON R. CREB-mediated Bcl-2 protein expression following ischemic preconditioning. 2005 Feb;25(2):234-46.
7. Dexi Chen, Manabu Minami, David C. Henshall; Roger P. Simon. Preconditioning Ischemia Enhances Base-Excision Repair Capability within Rat Brain Mitochondrial DNA. J Cereb Blood Flow Metab. 2003 Jan;23(1):88-98.
8. Dexi Chen?, Kunlin Jin*, Kenji Kawaguchi?, Masaki Nakayama?, Xiaoman Zhou?, Zhigang Xiong?, An Zhou?, David A. Greenberg*, Steven H. Graham?, and Roger P. Simon?. Ero1-L, an Ischemia-Inducible Gene from Rat Brain with Homology to Global Ischemia-Induced Gene 11 (Giig11), is Localized to Neuronal Dendrites by a Dispersed Identifier (ID)Element-Dependent Mechanism. J Neurochem. 2003 May;85(3):670-9
9. Dexi Chen, Guodong Cao, Cristine O’Horo, Wei Pei and Jun Chen. DNA Base-Excision Repair Activity for Oxidative Lesions in Rat Brain Mitochondria: Marked Age-dependent Regulation. J Neurochem 2002 81 1273-1284.
10. K. Jin1, X.O. Mao1, M.C. Eshoo1, G. del Rio1, R. Rao1 , D. Chen2, R.P. Simon2 and D.A. Greenberg.1cDNA Microarray Analysis of Changes in Gene Expression Induced by Neuronal Hypoxia In Vitro. Neurochem Res. 2002 Oct;27(10):1105-12.
11. Xiangping Chu; J. Jefferson; M. Johnson; L. Root; X. Zhu.; Dexi Chen; R. Simon; Z. Xiong. PC12 cells contain a singtle population of functional protein-gated channels-homomultimeric asic1A. J Neuroph 2002, 87 2555-256.
12. Martha B. Johnson, Manabu Minami, Dexi Chen, Roger P. Simon. Global ischemia induces expression of Asic2 in rat brain. J Cereb Blood Flow Metab. 2001, 21:734-740
13. Li YY, Chen D, Watkins SC, Feldman AM..Mitochondrial Abnormalities in Tumor Necrosis Factor-alpha-Induced Heart Failure Are Associated With Impaired DNA Repair Activity. Circulation. 2001 Nov 13;104(20):2492-7
14. Cao G, Minami M, Pei W, Yan C, Chen D, O'Horo C, Graham SH, Chen J. Intracellular Bax translocation after transient cerebral ischemia: implications for a role of the mitochondrial apoptotic signaling pathway in ischemic neuronal death.
J Cereb Blood Flow Metab. 2001 Apr;21(4):321-33.
15. Chen D, Stetler RA, Cao GD, Pei W, Yin X-M, Chen J. Characterization of the rat DFF35/ICADs: the endogenous inhibitor of DNA fragmentation in neuronal apoptosis. J Biol Chem. 2000 Dec 8;275(49):38508-17.
16. Chen D, Jing Lan, Wei Pei, Jun Chen. Detection of DNA Base-Excision repair activity for oxidative lesions in adult rat brain mitochondria. J Neurosci Res 2000. 61(2):225-236.
17. Yan C, Chen J, Chen D, Minami M, Pei W, Yin XM, Simon RP. Overexpression of the cell death suppressor Bcl-w in ischemic brain: implications for a neuroprotective role via the mitochondrial pathway. J Cereb Blood Flow Metab 2000 Mar;20(3):620-30.
18. Nagayama T, Lan J, Henshall DC, Chen D, O'Horo C, Simon RP, Chen J.Induction of oxidative DNA damage in the peri-infarct region after permanent focal cerebral ischemiaJ Neurochem 2000 Oct;75(4):1716-28
19. Nagayama T, Simon RP, Chen D, Henshall DC, Pei W, Stetler RA, Chen J .Activation of poly(ADP-ribose) polymerase in the rat hippocampus may contribute to cellular recovery following sublethal transient global ischemia. J Neurochem 2000 Apr;74(4):1636-45.
20. 转染野生型p53对乳腺癌细胞所致细胞凋亡的研究。 肿瘤(中文) 2000 Vol 20 P.100-102。马丽萍 陈德喜 王申五
21. 白血病细胞p16基因突变分析。中国实验血液学杂志2000 Vol.8 No.2 P.114-117。武莎莎 曹国栋 陈德喜 潘秀英 王申五 
22. bcl-2蛋白不能抑制VP16诱导的乳腺癌细胞调亡.中国肿瘤临床1999.13(1). P:6-7.陈德喜 马丽萍 曹国栋 王申五
23. 真核表达载体pcDNA-tPA的构建.哈尔滨医科大学学报1999.2. P:107-109 赵永波, 曹国栋, 陈德喜.
24. p53基因转导诱发慢性髓性白血病患者骨髓细胞凋亡. 中国实验血液学杂志1999.7(1).P:79-80. 马丽萍 童春荣 王申五 陈德喜
25. 转染bcl-2 基因抑制足叶乙甙诱导的K562 细胞凋亡. 北京医科大学学报1998.30(1) P35-37. 陈德喜 王申五 曹国栋.
26. 转导野生型p53基因提高K562细胞对紫外线诱导的细胞凋亡的敏感性. 中国实验血液学杂志 1998.6(3). P:224-226. 陈德喜 王申五 曹国栋.
27. 反义bcl-2—正义bcl-Xs逆转录病毒重组体的构建. 中国实验血液学杂志1998. 6(2).P.137-139. 曹国栋 王申五 陈德喜.
28. 逆转录病毒介导的反义Bcl-2序列促进足叶乙甙诱导的白血病细胞凋亡. 中华血液学杂志1998.9.P:467-469. 曹国栋 王申五 陈德喜
29. 介绍一种PCR鉴定重组体DNA插入方向及转染的方法. 中国生物化学与分子生物学报1998.14(6).P:757-760. 曹国栋 王申五 陈德喜
30. 化学发光杂交进行菌落克隆原位筛选。生物化学杂志,1997.5.P:537-539. 陈德喜,张宝春,王申五.
31. 一种快速RT-PCR法分析培养细胞基因表达水平. 北京医科大学学报 1997.3.P:280~282. 陈德喜,魏 妤,王申五
Presentation and Meeting
1. Dexi Chen, Zhiyong Yu, Zhiyi Zhu, Lopez CD. E2F target to XRCC1and contribution to single strand broken repair in S phase. Will be Presented in AACR 97th annual meeting, 2006, April 1 – 5 Washington DC
2. Dexi Chen , Zhiyi Zhu, Zhiyong Yu, Charles D. Lopez. An intact p53 pathway is required for efficient mitochondrial BER. American Association for Cancer Research annual meeting (96), April 16 – 20 2005. Los Angel
3. Dexi Chen, Zhiyi Zhu, Emerson Padiernos, Izidore S. Lossos, Charles D. Lopez. ASPP2/53BP2 is an E2F target gene and many link the p53 and RB/E2F pathways. American Association for Cancer Research annual meeting (95) March 27-31, 2004 Orlando, Florida.
4. Dexi Chen, Manabu Minami, Martha B. Johnson, Jing Quan Lan, Roger P. SimonThe induction of Bcl-2 expression by CREB and CBP contributes to neuronal survival in focal ischemic tolerance. Society for neuroscience annual meeting (31). San Diago, CA. 2001
5. Dexi Chen, Guodong Cao, Cristine O’Horo, Wei Pei and Jun Chen. DNA Base-Excision Repair Activity for Oxidative Lesions in Rat Brain Mitochondria: Marked Age-dependent Regulation, Society for neuroscience annual meeting (30). New Orleans, Louisiana. Nov, 4-9, 2000.
6. Guodong , Cao Dexi Chen, Cristine O’Horo, Wei Pei , Yougjie Liu and Jun Chen. Cloning and characterization of rat Caspase-9: implication for neuronal cell death after transient cerebral ischemia and cerebellar development. Society for neuroscience annual meeting (30). New Orleans, Louisiana. Nov, 4-9, 2000.
7. Guodong Cao, Dexi Chen, Roger, P.S. and Jun Chen. Molecular cloning and characterization of rat caspasae-activated deoxyribonuclease(CAD): Implication for neuronal cell death after transient cerebral ischemia. Society for neuroscience annual meeting (29). Miami Beach, Florida. Oct, 23-28,1999.
8. Dexi Chen,W Pei, DA Greenberg, S Irvin, A sinor, SH Granam, J Chen et al. Necrosis in primary cultures of cortical neurons following ischemia-like insults: Induction of DNA strand breaks as a death mediator. Society for neuroscience annual meeting(28). Cos Angeles, Calif . Nov. 7-12, 1998.
9. Jing Lan
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