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王顺启老师介绍
王顺启，男，1978年生，生命科学学院生物技术副教授、硕士生导师，研究方向为细胞分子生物学及中药学。1999年7月毕业于南开大学生物化学及分子生物学系，获理学学士学位，2004年7月毕业于南开大学生物系遗传学专业，获理学博士学位。2004年7月，在南昌大学生物技术系担任教师。2005年7月～2008年7月在南昌大学食品科学与工程中心从事博士后工作。
研究方向：
（1）天然中草药的组分分离、结构及活性鉴定研究。
（2）细胞应答外界刺激因子的分子生物机理研究。
主要特色：利用微波萃取，二氧化碳超临界，液相色谱，逆流色谱等技术进行中草药中天然成分的分离，借助于核磁共振，红外色谱等技术进行分离组分的结构鉴定。利用细胞学平台进行活性成分的筛选，并利用分子生物学的手段探讨细胞对部分天然中草药成分的应答机理。
目前承担的课题：
1．国家自然科学基金项目（项目批准号：30660266）：大粒车前子多糖调控树突状细胞功能的研究。
2．江西省教育厅项目(项目批准号：2007047) 独角莲抗癌活性成分的分离与鉴定。
3．中国博士后科学基金（资助号：20060390967），独角莲天然组分的筛选研究。
代表性论文或著作
2007，生物化学考研精解［M］（书号：978-7-03-019921-8），北京：科学出版社。本人承担副主编。
2007，中药材（国内刊号：CN 44-1286/R 国际刊号：ISSN 1001-4454）；白花蛇舌草对肝癌细胞SMMC-7721基因表达的影响［J］；王顺启，陈力，谢明勇，等。
2006，中草药（国内刊号：12-1108/R 国际刊号：ISSN 0253-2670）；独角莲水提取物对肝癌细胞SMMC-7721基因表达的影响［J］；王顺启，倪虹，王广良，等。
2006，南昌大学学报（理科版，国内代号：CN36-1193/N，国际刊号：ISSN 1006-0464）；《生物化学》多媒体教学课件制作［J］；毛慧玲，欧阳涟，王顺启，等。
2005，南开大学学报（自然科学版，国内刊号：12-1105/N国际刊号：ISSN 0465-7942）；应用抑制性消减杂交技术构建人肝癌组织特异表达cDNA文库［J］；王顺启，冯占宁，陈力，等。
2005，江西科学（国内刊号：CN 36-1093/N 国际刊号：ISSN 1001-3697）；真菌多糖的研究进展［J］；王小东，毛慧玲，王顺启。
2004，中国中药杂志（国内刊号：CN 11-2272/R国际刊号：ISSN 1001-5302）；mRNA差异显示法比较独角莲作用肝癌细胞SMMC-7721前后的基因表达［J］；王顺启，倪虹，程华，等；排名第一。
2004, International Biomedical Science Conference; Detection of differentially expressed genes in hepatocellular carcinoma cells SMMC-7721 responding to Typhonium giganteum Engl by gene microarray［C］; WANG Shun-qi, NI Hong, CHEN Li, et al。
2004, International Biomedical Science Conference; Detection of differentially expressed genes in hepatocellular carcinoma cells SMMC-7721 responding to Typhonium giganteum Engl by mRNA differential display［C］; WANG Shun-qi, NI Hong, CHEN Li, et al。
2003，细胞生物学杂志（国内刊号：CN 31-1478/Q国际刊号：ISSN 0253-9977）；独角莲对肝癌细胞SMMC-7721细胞增殖抑制作用机理的研究；王顺启，倪 虹，王 娟，等。
2003，天津中医药（国内刊号：CN 12-1349/R国际刊号：ISSN 1672-1519）；莪术挥发油抑制人肝癌细胞株SMMC-7721生长的实验研究［J］；王娟，王顺启，宋文芹，等。
Personal data:
Family Name: Wang First Name: Shunqi Gender: Male
Major: Genetics Marital Status: Married Birthday: 08/19/1978
Citizenship: P.R. China Health: Excellent Place of Birth: Fugou City, Henan Province
Graduate University: Nankai University Graduate Date: 07/2004 Degree: Ph.D.
E-mail: wangshunqi2004@hotmail.com, wangshunqi2004@sina.com.cn
Tel: 86-791-3989863 Fax: 86-791-8304347 Mobile Phone Number: 13870644581
Education:
l 09/1999 to present: Department of Genetics, College of Life Sciences, Nankai University, Ph.D..
l 09/1995 to 07/1999: Department of Biochemistry and Molecular, College of Life Sciences, Nankai University, Bachelor of Science.
l 07/2005-07/2008, post-doctor in Food and Technology State Key Laboratory of Nanchang University.
Research Experience:
l 01/1999-07/1999, Studies on the differential sexual genes of Ginkgobilobs, by RAPD (Randomly amplified Polymorphic DNA) and AFLP (Amplified Fragment Length Ploymorphism).
l 08/2000-01/2001, Studies on the chip of gene detection for hepatitis virus.
l 08/2000-01/2001, Studies on the chip of gene detection for TTV subspecies.
l 02/2001-12/2001, Make a condition for cell culture.
l 02/2001-01/2002, Separate and distill the ingredients of several Chinese traditional medicine.
l 08/2001-10/2002, Construct the library of complementary DNA of hepatocellular carcinoma by SSH (Suppression Subtractive Hybridization); then construct the microarray of highly expressed genes of hepatocellular carcinoma.
l 02/2002-03/2003, Studies on the mechanism of hepatocellular carcinoma cells SMMC-7721 responding to several Chinese traditional medicines.
l 02/2002-12/2003, Studies on the chip of hepatocellular carcinoma SNP (Single Nucleotide Polymorphism).
l 02/2002-04/2004, Studies on the mechanism of hepatocellular carcinoma cells SMMC-7721 responding to Typhonium giganteum Engl., from different levels and different respects, such as invert microscope, fluorescence microscope, MTT colorimetric assay, Flow Cytometry, microarray technology, DD RT-PCR (Differential Display Reverse Transcript PCR), Northern bolt, etc..
l 02/2001-07/2001, assistant in cell biology experiment in two classes (30 students per class).
l 07/2004- , associate professor in Institute of Life Science of Nanchang University.
Publications:
1. Wang Shunqi, Chen Li, Ni Hong, Xie Mingyong The Effects of genes expression on hepatocarcinoma cell SMMC-7721 responding to Hedyotis diffusa Wild Journal of Chinese Medicinal Materials.2007, 30(10) (in Publication).
Abstract: ［Objective］ To detect the effects of Hedyotis diffusa Wild (HDW) on the growth and gene expression of hepatocarcinoma cell SMMC-7721. ［Method］ The growth effect of hepatocarcinoma cell SMMC-7721 responding to HDW was detected by MTT(monotetrazoluim), and the gene expression change of hepatocarcinoma cell SMMC-7721 responding to HDW for 36 hours was explored with microarray of hepatocarcinoma associated genes, which resulted from suppression subtractive hybridization library. ［Result］ After hepatocarcinoma cell SMMC-7721 was treated by HDW for 36 hours, the analysis of gene expression profiles indicated that 1 genes expression were up-regulated and 14 genes expression were down-regulated. ［Conclusion］ The present study conclude that hepatocarcinoma cells can be affected by HDW through changes of multi-genes expression. The research provided important clues to the molecular mechanism how hepatocarcinoma cells to response to HDW.
2. WANG Shun-qi, NI Hong, WANG Guang-liang, CHENG Hua, LI Hua-bing, CHEN Li. Detection of Differentially Expressed Genes in Hepatocellular Carcinoma Cells SMMC-7721 responding to Typhonium giganteum Engl. by using hepatocarcinoma expression gene microarray. Chinese Traditional and Herbal Drugs. 2006, 37(9):1384-1387.
Abstract: Objective To detect differentially expressed genes in hepatocellular carcinoma cells SMMC-7721 responding to Typhonium giganteum Engl.. Methods The response of hepatocellular carcinoma cells SMMC-7721 to the aqueous extract from dried powdered rhizomes of Typhonium giganteum Engl.(AEoTGE) for 36 hours was explored with microarray of hepatocarcinoma expression genes which resulted from suppression subtractive hybridization library. Results After hepatocarcinoma cells SMMC-7721 were treated by AEoTGE for 36 hours, the analysis of gene expression profiles indicated that 16 genes expression were up-regulated and 2 genes expression were down-regulated. Conclusion The present study conclude that hepatocarcinoma cells can be affected by Typhonium giganteum Engl. through changes of multi-genes expression. The research provided important clues to the molecular mechanism how hepatocarcinoma cells to response to AEoTGE, and useful genes that isolate potent ingredient of Typhonium giganteum Engl..
3. WANG Shunqi, FENG Zhanning, WANG Guangliang, Chenghua, Wang Tongshun, NI Hong, SONG Wenqin, CHEN Li. GENERATION AND ANALASIS OF A DIFFERENTIALLY EXPRESS GENES cDNA LIBRARY OF HUMAN PRIMARY HEPATOCELLULAR CARCINOMA. Acta Scientiarum Naturalium Universitatis Nankaiensis. 2005, 38(4):115-118.
Abstract: In order to generate a differentially express genes cDNA library of human hepatocellular carcinoma. The cDNA fragments of differentially expressed genes between hepatocellular carcinoma (HCC) tissue and paracancerous liver tissues were isolated by suppression subtractive hybridization (SSH). After amplified with PCR，the cDNA fragments were cloned. As the result, the SSH library, we obtained, contained 1820 positive clones. 98 percent of positive clones were 100-800bp insert-fragments. The sequence analysis of 260 cDNA fragments has proved that these fragments were tightly correlated with the occurrence and development of the hepatocellular carcinoma. These results suggest a high quality and high representative cDNA library of human hepatocellular carcinoma was constructed successfully.
4. WANG Xiao-dong, MAO Hui-ling, WANG Shun-qi. Research Advance ment of fungus poslysaccharides. Jiangxi Science. 2005, 23(3):243-246,280
5. WANG Shun-qi, NI Hong, CHENG Hua, WANG Guang-liang, LI Hua-bing, WANG Tong-shun, CHEN Li. Detection of differentially expressed genes in hepatocellular carcinoma cells SMMC-7721 responding to Typhonium giganteum Engl. by mRNA differential display. China Journal of Chinese Materia Medica, 2004, 29(10):974-977.
Abstract: Objective To screen and identify the differentially expressed genes in hepatocellular carcinoma cells SMMC-7721 responding to the aqueous extract from dried powdered rhizomes of Typhonium giganteum Engl.(AEoTGE) . Method The response of hepatocellular carcinoma cells SMMC-7721 to AEoTGE for 36 hours was explored with the technique of mRNA differential display. Result After hepatocarcinoma cells SMMC-7721 were treated by AEoTGE for 36 hours，it indicated that 1 gene expression is upgrade and 1 gene expression is downgrade induced by AEoTGE. Conclusion The research provided important clues to the molecular mechanism how hepatocarcinoma cells to response to Typhonium giganteum Engl..
6. WANG Shun Qi, NI Hong, WANG Juan, CHEN Li. Studies on the proliferation restrain of Typhonium giganteum Engl. to Hepatocarcinoma Cell SMMC-7721. Chinese Journal of Cell Biology, 2003, 25(3): 185-188.
Abstract: Typhonium giganteum Engl. has been used to treat many cancers for many years in China. This research was designed to study the inhibition of the aqueous extract from dried powdered rhizomes of Typhonium giganteum Engl.(AEoTGE) on the proliferation, cell cycle and apoptosis of hepatocarcinoma cell SMMC-7721. MTT colorimetric assay and flow cytometry results showed: AEoTGE can decrease the proliferation rate of SMMC-7721 cell; the significant S phase arrest was revealed by the increase of cells in S phase and the obvious apoptosis of SMMC-7721 cell was induced by AEoTGE. Taken together, these results suggest that Typhonium giganteum Engl. is a promising herbal drug in the therapy of hepatocellular carcinoma.
7. Wang Juan, Wang Shunqi, Ni Hong, Chen Li. Experimental research of Ezhu (turmeric rhizome) naphtha on inhibiting in growth of human carcinoma cells SMMC-7721. Tian journal of Traditional Chinese Medicine. 2003, 20(1): 48-51.
Abstract: Objective To discuss the inhibition mechanism of turmeric rhizome naphtha on inhibition in the growth of human carcinoma cells SMMC-7721. Method Fluorescence microscope was used to observe the cytomorphology changes before and after giving the turmeric rhizome naphtha. The inhibition effect was detected by (monofetrazolium) MTT (monotetrazoluim) and the cells' cyclic retardation and apoptosis rate were examined by using flow cytometry. Result MTT test showed that the concentration of turmeric rhizome naphtha had a direct correlation with the inhibiting rate of cell growth. The result of fluorescence microscope showed that 24h after administration turmeric rhizome naphtha could 28.155 and cell cycle was arrested at S phase. Conclusion Turmeric rhizome naphtha can inhibit the growth of hepatocell SMMC-7721, Inducing apoptosis and blocking the cell cycle are the important mechanism of inhibition effect.
8. Wang Shunqi, Feng Zhanning, Liu Guohong, Wang Guangliang, Ni Hong, Chen Li. Construction and analysis of the suppression subtractive hybridization library of liver cancer. The Eighth National Cell Biological Discussion (summary, 2003.11)
9. Wang Shunqi, Ni Hong, Wang Juan, Chen Li. Discussion about the mechanism of proliferation restrain of Typhonium giganteum Engl. to Hepatocarcinoma Cell SMMC-7721. The Eighth National Cell Biological Discussion (summary, 11/2003).
10. Zhang Rongxin, Zeng Hui, Wang Shunqi, et al. Assay of DNA homology between A chromosome and B chromosome in maize by AFLP. The Seventh National Cell Biological Discussion (summary, 05/1999).
研究方向：
（1）天然中草药的组分分离、结构及活性鉴定研究。
（2）细胞应答外界刺激因子的分子生物机理研究。
主要特色：利用微波萃取，二氧化碳超临界，液相色谱，逆流色谱等技术进行中草药中天然成分的分离，借助于核磁共振，红外色谱等技术进行分离组分的结构鉴定。利用细胞学平台进行活性成分的筛选，并利用分子生物学的手段探讨细胞对部分天然中草药成分的应答机理。
目前承担的课题：
1．国家自然科学基金项目（项目批准号：30660266）：大粒车前子多糖调控树突状细胞功能的研究。
2．江西省教育厅项目(项目批准号：2007047) 独角莲抗癌活性成分的分离与鉴定。
3．中国博士后科学基金（资助号：20060390967），独角莲天然组分的筛选研究。
代表性论文或著作
2007，生物化学考研精解［M］（书号：978-7-03-019921-8），北京：科学出版社。本人承担副主编。
2007，中药材（国内刊号：CN 44-1286/R 国际刊号：ISSN 1001-4454）；白花蛇舌草对肝癌细胞SMMC-7721基因表达的影响［J］；王顺启，陈力，谢明勇，等。
2006，中草药（国内刊号：12-1108/R 国际刊号：ISSN 0253-2670）；独角莲水提取物对肝癌细胞SMMC-7721基因表达的影响［J］；王顺启，倪虹，王广良，等。
2006，南昌大学学报（理科版，国内代号：CN36-1193/N，国际刊号：ISSN 1006-0464）；《生物化学》多媒体教学课件制作［J］；毛慧玲，欧阳涟，王顺启，等。
2005，南开大学学报（自然科学版，国内刊号：12-1105/N国际刊号：ISSN 0465-7942）；应用抑制性消减杂交技术构建人肝癌组织特异表达cDNA文库［J］；王顺启，冯占宁，陈力，等。
2005，江西科学（国内刊号：CN 36-1093/N 国际刊号：ISSN 1001-3697）；真菌多糖的研究进展［J］；王小东，毛慧玲，王顺启。
2004，中国中药杂志（国内刊号：CN 11-2272/R国际刊号：ISSN 1001-5302）；mRNA差异显示法比较独角莲作用肝癌细胞SMMC-7721前后的基因表达［J］；王顺启，倪虹，程华，等；排名第一。
2004, International Biomedical Science Conference; Detection of differentially expressed genes in hepatocellular carcinoma cells SMMC-7721 responding to Typhonium giganteum Engl by gene microarray［C］; WANG Shun-qi, NI Hong, CHEN Li, et al。
2004, International Biomedical Science Conference; Detection of differentially expressed genes in hepatocellular carcinoma cells SMMC-7721 responding to Typhonium giganteum Engl by mRNA differential display［C］; WANG Shun-qi, NI Hong, CHEN Li, et al。
2003，细胞生物学杂志（国内刊号：CN 31-1478/Q国际刊号：ISSN 0253-9977）；独角莲对肝癌细胞SMMC-7721细胞增殖抑制作用机理的研究；王顺启，倪 虹，王 娟，等。
2003，天津中医药（国内刊号：CN 12-1349/R国际刊号：ISSN 1672-1519）；莪术挥发油抑制人肝癌细胞株SMMC-7721生长的实验研究［J］；王娟，王顺启，宋文芹，等。
Personal data:
Family Name: Wang First Name: Shunqi Gender: Male
Major: Genetics Marital Status: Married Birthday: 08/19/1978
Citizenship: P.R. China Health: Excellent Place of Birth: Fugou City, Henan Province
Graduate University: Nankai University Graduate Date: 07/2004 Degree: Ph.D.
E-mail: wangshunqi2004@hotmail.com, wangshunqi2004@sina.com.cn
Tel: 86-791-3989863 Fax: 86-791-8304347 Mobile Phone Number: 13870644581
Education:
l 09/1999 to present: Department of Genetics, College of Life Sciences, Nankai University, Ph.D..
l 09/1995 to 07/1999: Department of Biochemistry and Molecular, College of Life Sciences, Nankai University, Bachelor of Science.
l 07/2005-07/2008, post-doctor in Food and Technology State Key Laboratory of Nanchang University.
Research Experience:
l 01/1999-07/1999, Studies on the differential sexual genes of Ginkgobilobs, by RAPD (Randomly amplified Polymorphic DNA) and AFLP (Amplified Fragment Length Ploymorphism).
l 08/2000-01/2001, Studies on the chip of gene detection for hepatitis virus.
l 08/2000-01/2001, Studies on the chip of gene detection for TTV subspecies.
l 02/2001-12/2001, Make a condition for cell culture.
l 02/2001-01/2002, Separate and distill the ingredients of several Chinese traditional medicine.
l 08/2001-10/2002, Construct the library of complementary DNA of hepatocellular carcinoma by SSH (Suppression Subtractive Hybridization); then construct the microarray of highly expressed genes of hepatocellular carcinoma.
l 02/2002-03/2003, Studies on the mechanism of hepatocellular carcinoma cells SMMC-7721 responding to several Chinese traditional medicines.
l 02/2002-12/2003, Studies on the chip of hepatocellular carcinoma SNP (Single Nucleotide Polymorphism).
l 02/2002-04/2004, Studies on the mechanism of hepatocellular carcinoma cells SMMC-7721 responding to Typhonium giganteum Engl., from different levels and different respects, such as invert microscope, fluorescence microscope, MTT colorimetric assay, Flow Cytometry, microarray technology, DD RT-PCR (Differential Display Reverse Transcript PCR), Northern bolt, etc..
l 02/2001-07/2001, assistant in cell biology experiment in two classes (30 students per class).
l 07/2004- , associate professor in Institute of Life Science of Nanchang University.
Publications:
1. Wang Shunqi, Chen Li, Ni Hong, Xie Mingyong The Effects of genes expression on hepatocarcinoma cell SMMC-7721 responding to Hedyotis diffusa Wild Journal of Chinese Medicinal Materials.2007, 30(10) (in Publication).
Abstract: ［Objective］ To detect the effects of Hedyotis diffusa Wild (HDW) on the growth and gene expression of hepatocarcinoma cell SMMC-7721. ［Method］ The growth effect of hepatocarcinoma cell SMMC-7721 responding to HDW was detected by MTT(monotetrazoluim), and the gene expression change of hepatocarcinoma cell SMMC-7721 responding to HDW for 36 hours was explored with microarray of hepatocarcinoma associated genes, which resulted from suppression subtractive hybridization library. ［Result］ After hepatocarcinoma cell SMMC-7721 was treated by HDW for 36 hours, the analysis of gene expression profiles indicated that 1 genes expression were up-regulated and 14 genes expression were down-regulated. ［Conclusion］ The present study conclude that hepatocarcinoma cells can be affected by HDW through changes of multi-genes expression. The research provided important clues to the molecular mechanism how hepatocarcinoma cells to response to HDW.
2. WANG Shun-qi, NI Hong, WANG Guang-liang, CHENG Hua, LI Hua-bing, CHEN Li. Detection of Differentially Expressed Genes in Hepatocellular Carcinoma Cells SMMC-7721 responding to Typhonium giganteum Engl. by using hepatocarcinoma expression gene microarray. Chinese Traditional and Herbal Drugs. 2006, 37(9):1384-1387.
Abstract: Objective To detect differentially expressed genes in hepatocellular carcinoma cells SMMC-7721 responding to Typhonium giganteum Engl.. Methods The response of hepatocellular carcinoma cells SMMC-7721 to the aqueous extract from dried powdered rhizomes of Typhonium giganteum Engl.(AEoTGE) for 36 hours was explored with microarray of hepatocarcinoma expression genes which resulted from suppression subtractive hybridization library. Results After hepatocarcinoma cells SMMC-7721 were treated by AEoTGE for 36 hours, the analysis of gene expression profiles indicated that 16 genes expression were up-regulated and 2 genes expression were down-regulated. Conclusion The present study conclude that hepatocarcinoma cells can be affected by Typhonium giganteum Engl. through changes of multi-genes expression. The research provided important clues to the molecular mechanism how hepatocarcinoma cells to response to AEoTGE, and useful genes that isolate potent ingredient of Typhonium giganteum Engl..
3. WANG Shunqi, FENG Zhanning, WANG Guangliang, Chenghua, Wang Tongshun, NI Hong, SONG Wenqin, CHEN Li. GENERATION AND ANALASIS OF A DIFFERENTIALLY EXPRESS GENES cDNA LIBRARY OF HUMAN PRIMARY HEPATOCELLULAR CARCINOMA. Acta Scientiarum Naturalium Universitatis Nankaiensis. 2005, 38(4):115-118.
Abstract: In order to generate a differentially express genes cDNA library of human hepatocellular carcinoma. The cDNA fragments of differentially expressed genes between hepatocellular carcinoma (HCC) tissue and paracancerous liver tissues were isolated by suppression subtractive hybridization (SSH). After amplified with PCR，the cDNA fragments were cloned. As the result, the SSH library, we obtained, contained 1820 positive clones. 98 percent of positive clones were 100-800bp insert-fragments. The sequence analysis of 260 cDNA fragments has proved that these fragments were tightly correlated with the occurrence and development of the hepatocellular carcinoma. These results suggest a high quality and high representative cDNA library of human hepatocellular carcinoma was constructed successfully.
4. WANG Xiao-dong, MAO Hui-ling, WANG Shun-qi. Research Advance ment of fungus poslysaccharides. Jiangxi Science. 2005, 23(3):243-246,280
5. WANG Shun-qi, NI Hong, CHENG Hua, WANG Guang-liang, LI Hua-bing, WANG Tong-shun, CHEN Li. Detection of differentially expressed genes in hepatocellular carcinoma cells SMMC-7721 responding to Typhonium giganteum Engl. by mRNA differential display. China Journal of Chinese Materia Medica, 2004, 29(10):974-977.
Abstract: Objective To screen and identify the differentially expressed genes in hepatocellular carcinoma cells SMMC-7721 responding to the aqueous extract from dried powdered rhizomes of Typhonium giganteum Engl.(AEoTGE) . Method The response of hepatocellular carcinoma cells SMMC-7721 to AEoTGE for 36 hours was explored with the technique of mRNA differential display. Result After hepatocarcinoma cells SMMC-7721 were treated by AEoTGE for 36 hours，it indicated that 1 gene expression is upgrade and 1 gene expression is downgrade induced by AEoTGE. Conclusion The research provided important clues to the molecular mechanism how hepatocarcinoma cells to response to Typhonium giganteum Engl..
6. WANG Shun Qi, NI Hong, WANG Juan, CHEN Li. Studies on the proliferation restrain of Typhonium giganteum Engl. to Hepatocarcinoma Cell SMMC-7721. Chinese Journal of Cell Biology, 2003, 25(3): 185-188.
Abstract: Typhonium giganteum Engl. has been used to treat many cancers for many years in China. This research was designed to study the inhibition of the aqueous extract from dried powdered rhizomes of Typhonium giganteum Engl.(AEoTGE) on the proliferation, cell cycle and apoptosis of hepatocarcinoma cell SMMC-7721. MTT colorimetric assay and flow cytometry results showed: AEoTGE can decrease the proliferation rate of SMMC-7721 cell; the significant S phase arrest was revealed by the increase of cells in S phase and the obvious apoptosis of SMMC-7721 cell was induced by AEoTGE. Taken together, these results suggest that Typhonium giganteum Engl. is a promising herbal drug in the therapy of hepatocellular carcinoma.
7. Wang Juan, Wang Shunqi, Ni Hong, Chen Li. Experimental research of Ezhu (turmeric rhizome) naphtha on inhibiting in growth of human carcinoma cells SMMC-7721. Tian journal of Traditional Chinese Medicine. 2003, 20(1): 48-51.
Abstract: Objective To discuss the inhibition mechanism of turmeric rhizome naphtha on inhibition in the growth of human carcinoma cells SMMC-7721. Method Fluorescence microscope was used to observe the cytomorphology changes before and after giving the turmeric rhizome naphtha. The inhibition effect was detected by (monofetrazolium) MTT (monotetrazoluim) and the cells' cyclic retardation and apoptosis rate were examined by using flow cytometry. Result MTT test showed that the concentration of turmeric rhizome naphtha had a direct correlation with the inhibiting rate of cell growth. The result of fluorescence microscope showed that 24h after administration turmeric rhizome naphtha could 28.155 and cell cycle was arrested at S phase. Conclusion Turmeric rhizome naphtha can inhibit the growth of hepatocell SMMC-7721, Inducing apoptosis and blocking the cell cycle are the important mechanism of inhibition effect.
8. Wang Shunqi, Feng Zhanning, Liu Guohong, Wang Guangliang, Ni Hong, Chen Li. Construction and analysis of the suppression subtractive hybridization library of liver cancer. The Eighth National Cell Biological Discussion (summary, 2003.11)
9. Wang Shunqi, Ni Hong, Wang Juan, Chen Li. Discussion about the mechanism of proliferation restrain of Typhonium giganteum Engl. to Hepatocarcinoma Cell SMMC-7721. The Eighth National Cell Biological Discussion (summary, 11/2003).
10. Zhang Rongxin, Zeng Hui, Wang Shunqi, et al. Assay of DNA homology between A chromosome and B chromosome in maize by AFLP. The Seventh National Cell Biological Discussion (summary, 05/1999).
王顺启老师相关教学资源
- 南昌大学教学资源
- 王顺启老师课程教学资源
